The story begins in 16th Century Spain as Conquistador Tomas Creo (Hugh Jackman) is sent on a holy mission by Queen Isabel (Rachel Weisz) to locate the Tree Of Life, which is believed to grant immortality to anyone who drinks of its sap. With only the aid of a cryptic map in the form of an ancient Mayan dagger, will Creo discover the key to everlasting life and save New Spain and its Queen?